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These results with fresh non fractionated not
2018-10-24
These results with fresh, non-fractionated, not culture-expanded, adipose tissue-derived SVF are comparable with other two previously reported clinical series, using cultured adipose-derived ADSCs with controlled cell doses. The adipose tissue contains large populations of mesenchymal glibenclamide
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br Pluripotent stem cells from non
2018-10-24
Pluripotent stem cells from non-rodent mammals acquire a more downstream potency level Successful derivation of preimplantation embryo-derived pluripotent stem cell lines was reported for rabbit [Oryctolagus cuniculus] (Honda et al., 2008; Osteil et al., 2013; Osteil et al., 2016b; Wang et al., 2
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br Heteroplasmy mitochondrial segregation and disease
2018-10-24
Heteroplasmy, mitochondrial segregation and disease In an individual with a mitochondrial disease, different cells or populations may have differing amounts of the mutant mitochondria, ranging from complete homoplasmy – individuals with all mutant mitochondrial DNA as occurs in Leigh\'s Syndrome
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As an initial application of our RNA seq datasets
2018-10-24
As an initial application of our RNA-seq datasets for discovery of regulatory mechanisms in huSCs, we compared transcriptomes of quiescent huSCs to those of activated huSCs to identify loci that were differentially expressed. In quiescent huSCs, we identified 998 highly expressed genes, including 13
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br Results br Discussion The identification of
2018-10-24
Results Discussion The identification of CD13 and ROR2 as early cardiogenic markers contributes to the establishment of a detailed cardiac lineage fate map and may subsequently aid developmental studies. Other cell-surface markers for isolation of mesoderm and cardiac progenitors have previous
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Another question is the heterogeneity within and between
2018-10-24
Another question is the heterogeneity within and between organoids, which seems common to all the protocols developed so far but has not yet been studied in detail. Several processes, such as progenitor proliferation, cell differentiation, and ontogenetic cell death, could be potential sources of or
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A variety of studies have compared the expression profiles
2018-10-24
A variety of studies have compared the expression profiles, pluripotentiality, and genetic and epigenetic stability of hESC and iPSC including lines generated using different strategies, distinct parental somatic cell types, or reprogramming methods (Bock et al., 2011; International Stem Cell Initia
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We have developed protocols to form
2018-10-24
We have developed protocols to form 3D force-generating engineered heart tissues (EHTs; Eschenhagen et al., 2012; Hirt et al., 2014). This follows the principle of hydrogel formation with dissociated cardiomyocytes in casting molds and maintenance with a defined preload. Cardiomyocytes remodel the h
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The results presented here are consistent with the propositi
2018-10-24
The results presented here are consistent with the proposition that the onset of cellular pathology in FTD occurs earlier than clinical manifestations, which has been corroborated by biomarker studies in Alzheimer’s disease (Jack et al., 2013). By implementing a panel of biochemical and cellular as
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MET is an important step in reprogramming
2018-10-24
MET is an important step in reprogramming fibroblasts to iPSCs (Li et al., 2010; Samavarchi-Tehrani et al., 2010), and the acquisition of epithelial features and loss of mesenchymal features are known to occur soon after transfection with Yamanaka factors (David and Polo, 2014; Polo et al., 2012). T
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br Experimental Procedures br Author Contributions br Acknow
2018-10-24
Experimental Procedures Author Contributions Acknowledgments We thank A. Villanueva, C. Saura, C. Cruz, A. Fernández, E. Nadal, M. Martin, and R. Iggo for reagents and helpful advice, A. Welm and Y. DeRose for sharing PDX models, V. Peg, X. Serres, J. Balmaña, J. Pérez, and C. Hierro for pr
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The ability of antigen uptake
2018-10-24
The ability of antigen uptake in T-iPS-DCs was equal to that of mo-DCs, but there was a slight difference in cytokine production. T-iPS-DCs produced much more IL-6 and TNF-α than mo-DCs, especially when cultured with CD40L stimulation, but much less with other stimulations. T-iPS-DCs had a tendency
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br Summary The newly developed analyzer Soffron
2018-10-24
Summary The newly developed analyzer Soffron60 is designed for operation with GESA electron-beam material-treatment facilities [1] and can be used at machines with similar characteristics (∼60 kV, ∼100 A, ∼100 µs, 0.1 T) or even with more energetic beam. The analyzer allows determination of Calci
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Hosoya proposed that the cosmic time
2018-10-24
Hosoya [9] proposed that the cosmic time during the inflationary phase in slow-roll of the inflaton scalar field ϕ with the potential could be the inflaton field itself (or, to be precise, its logarithm, t ∼ ln (ϕ/ϕ0), where ϕ0 is the initial value of the field). In this regime, the master wave eq
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br This result is not
2018-10-24
This result is not unexpected, as the Crocco Eq. (3) is equivalent to a canonical system. A similar result is obtained when splitting series in the case of the Blasius equation: which is not connected to any variational problems. Furthermore, let y be and the split linear system takes the form
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