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Previous studies have demonstrated that the signal transduct
2018-11-08
Previous studies have demonstrated that the signal transduction pathways mediating ATF3 expression include c-Jun co-expressed with ATF3 in the nervous system after injury and stress (Hunt et al., 2012). The selective JNK/c-Jun inhibitor SP600125 fully blocks ATF3 induction in adult rat ganglion neur
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br Materials and methods br Results To easily quantify hemat
2018-11-08
Materials and methods Results To easily quantify hematopoietic development from human ES cells, we created a fluorescent reporter line. The pan-hematopoietic surface marker, CD43 (leukosialin/SPN), is expressed in all hematopoietic lineages beginning at the hematopoietic progenitor stage durin
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Several cytokines and chemokines possess important chemo att
2018-11-08
Several cytokines and chemokines possess important chemo-attractant functions during fetal CNS development (Stumm and Hollt, 2007; Li and Ransohoff, 2008). This suggests that neural stem and precursor dihydrofolate reductase may respond to cues by these mediators of inflammation in immune-mediated p
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Peripheral blood mononuclear cells PBMCs
2018-11-08
Peripheral blood mononuclear Motilin Receptor (PBMCs) were obtained from a 43-year-old male with familial HCM. The patient fulfilled clinical criteria for HCM with a maximum left ventricular wall thickness of 28mm (normal 7–10mm) in the absence of loading conditions. The patient is heterozygous for
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In this study we have shown that the human promoter
2018-11-08
In this study, we have shown that the human order ambroxol hydrochloride can be used to generate stable hPSC lines to report on GFAP-expressing astrocytes. Furthermore, we have shown that purification of fluorescently labeled astrocytes enables enrichment of regionally defined GFAP+ astrocytes. Th
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To confirm iPS identity and purity we checked embryonic
2018-11-08
To confirm iPS identity and purity, we checked embryonic stem cell makers, Tra-1-81 (Fig. 2A) and Tra-1-60 (not shown) by live staining and found all EZ Cap Reagent AG (3\' OMe) are Tra-1-81 positive. Acknowledgment The project described was supported by Award Number I21BX002215 (WX) from the B
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Placental growth factor PlGF is an angiogenic protein often
2018-11-08
Placental growth factor (PlGF) is an angiogenic protein often highly upregulated in solid tumors contributing to their growth and survival (Kim et al., 2012). PlGF can promote angiogenesis either by directly stimulating endothelial cell growth or by recruiting pro-angiogenic cell types. Of the two m
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We became interested in the potential role of ADF genes
2018-11-08
We became interested in the potential role of ADF genes and BQ-788 sodium salt dynamics during osteoblast (OB) differentiation of hMSCs for several reasons. First, actin and microtubules are abundant in osteoblasts (Aubin et al., 1983; Arena et al., 1991). During OB differentiation, cell morphology
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br Materials and methods br Results br Discussion br
2018-11-08
Materials and methods Results Discussion Conclusion Author contributions Acknowledgments This study was supported by a grant from the Innovative Research Institute for Cell Therapy (A062260) and supported by the a grant of the Korea Health Technology R&D Project through the Korea H
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In the present study we
2018-11-08
In the present study, we have used a set of OSCC cell lines and isolated the putative stem cell fraction (CD44highCD24low) along with CD44highCD24high and CD44lowCD24high (non-stem) sub-populations. These populations were subjected to various molecular and cellular assays to determine whether CD24 h
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Indeed both hAT MSC and hiPS
2018-11-08
Indeed, both hAT-MSC and hiPS-MSC baACL implants, when validated in a swine ACL excision model, resulted in proper ACL tissue formation. In the osseous zones, both in the tibia and the femur, the fibres of the biomaterial were surrounded by newly formed tissue that was encapsulated within mature bon
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Differentiation potential of the MMD ISCH iPSC line was
2018-11-08
Differentiation potential of the MMD ISCH#6 iPSC line was assessed by Embryoid Body assay. Differentiated 14-day old cultures exhibited the presence of β3-Tubulin (ectoderm), α-SMA (mesoderm) and TROMA-1 (endoderm) immunopositive mtor pathway (Fig. 2A), thus confirming the pluripotent nature of the
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br Material methods br Results br
2018-11-08
Material & methods Results Discussion Acknowledgements We thank Melissa Carballosa-Gautam in the Miami Project to Cure Paralysis Imaging Core. This design, experimentation, data analysis and manuscript writing was supported by the Miami Project to Cure Paralysis; NIH/NINDS (DJL: NS049545
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We herein show that simple supplementation with
2018-11-08
We herein show that simple supplementation with doxycycline greatly improves both hESC/iPSC viability and self-renewal. In practice, doxycycline dramatically enhances hESC/iPSC expandability, and the effects continue for long time periods. The effects of doxycycline are mediated by direct activation
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br Experimental Procedures br Author
2018-11-08
Experimental Procedures Author Contributions Acknowledgments Introduction Recently, substantial progress has been made to elicit organogenesis in 3D culture (Antonica et al., 2012; Huch et al., 2013; Liu et al., 2010; Sato et al., 2009; Spence et al., 2011). In the neural lineage, embryo
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